NUCLEUS

Developer Notes

Build synthetic cells that work

Developer Notes (DevNotes) are a short form of scientific communication that can be described in one sentence. They provide the Nucleus community with a means to rapidly share useful results and insights. Since they are built on top of Jupyter Notebooks they can easily integrate code and a variety of file types, bundling an entire research workflow into a discrete package. Continuing developments made to the Nucleus Distribution by the Core Development Team at b.next or by visiting residents and fellows at Nucleus Labs will be reported here before being integrated into the Distribution.

If you work on synthetic cells and related systems and/or work with the Nucleus Distribution, you are invited to submit a DevNote. DevNotes are open access and open source. By contributing a DevNote you can contribute to the development of the Nucleus Distribution. Results and insights that are highly useful and/or relevant will be integrated into the Nucleus Distribution by the Core Development Team at b.next.

Core

Integrating PPK Module in PURE Cells

PPK Module increases protein expression in PURE Cell
DevNote
Sep 7, 2025

Nucleus OnePot PURE Replication

Work in progress to replicate and improve upon the Nucleus OnePot PURE protocol
DevNote
Jul 28, 2025

The Developer Cell Control Module: Protein Degradation by ClpXP

The Developer Cell Control Module: Protein Degradation by ClpXP
DevNote
Jul 17, 2025

PPK Module testing in PURE

PPK Module increases protein expression
DevNote
Jul 15, 2025

Developer Cell: Project Introduction

Developer Cell: Project Introduction
DevNote
Jul 14, 2025

MTHFS - an Unexpected Enzyme in PURE

Why Folinic Acid Needs Extra Help
DevNote
Mar 13, 2025

Community